Review of NS1 and IgG/IgM Dengue Immunochromatography Test Results on Total Platelet Count and Platelet Index in Patients with Dengue Fever
DOI:
https://doi.org/10.32382/medkes.v20i1.1439Keywords:
Dengue Fever, Dengue IgG IgM, Dengue NS1, Platelet Count, Platelet IndeksAbstract
Dengue fever carries a risk of developing dengue shock syndrome, which can be fatal if not diagnosed and treated promptly. In addition to taking a medical history and performing a physical examination, laboratory tests are required. Laboratory tests that can be conducted include dengue NS1 testing, dengue IgG and IgM testing, and platelet count and platelet index measurements. This study aims to determine the correlation between NS1 and dengue IgG IgM test results and total platelet count and platelet indices (MPV, PDW, P-LCR, and PCT) in patients with dengue fever. This study is a correlational study using a cross-sectional analytical approach, employing purposive sampling techniques, and obtained 40 samples that met the study criteria. The study was conducted at the Clinical Pathology Laboratory of Hasanuddin University Hospital in Makassar from December 1, 2024, to February 28, 2025. The results of the study showed that there was a significant correlation between dengue IgG test results and total platelet count (p=0.032, p<0.05) and PDW (p=0.031, p<0.05), while there was no significant correlation with PCT test results (p=0.788, p>0.05). For dengue IgM and NS1 dengue test results, no significant correlation was found with total platelet count (p=0.565, p=0.624, p>0.05), PDW (p=0.728, p=0.188, p>0.05), and PCT (p=0.841, p=0.454 (p > 0.05). The results of MPV and P-LCR tests showed consistent results, so no statistical analysis was performed. It was concluded that total platelet count and platelet index PDW could serve as potential laboratory biomarkers for screening, diagnosis, and monitoring of dengue-infected patients based on dengue IgG test results.
Keywords: Dengue Fever; Dengue IgG IgM; Platelet Index; Platelet Count; Dengue NS1
References
Lorenza. A, Arkhaesi. N, and Hardian. H, “Comparison of Platelet Large Cell Ratio (P-LCR) in Children with Dengue Fever and Dengue Hemorrhagic Fever.” J Kedokt Diponegoro (Diponegoro Med Journal), (2018).
WHO, Dengue and Severe Dengue. (2022).
Ministry of Health of the Republic of Indonesia. Latest DHF Case Data in Indonesia. (2022).
South Sulawesi Provincial Health Office. Health Profile of South Sulawesi Province. (2020).
Aziz. K, “Relationship of Type of Infection with Platelet and Hematocrit Examination in Dengue Infection Patients at Urip Sumoharjo Hospital Bandar Lampung.” (2019).
Winarti. N, “Relationship between Dengue NS1 and Dengue IgG and IgM in Patients with Suspected Dengue Hemorrhagic Fever.” 5(3), 248–253, (2020).
Noreka. A. H, Lardo. S, and Nugrohowati. N, “Hematocrit, Thrombocyte, Body Mass Index, and Their Associations with the Severity of Dengue Hemorrhagic Fever Among Adult Patients at Esnawan Antariksa Air Force Hospital.” Jakarta, 640–643, Atlantis Press, (2020), https://doi.org/10.2991/ahsr.k.200215.125.
Astuti. D, “Platelet Index Value as Quality Control of Platelet Concentrate Components.” Meditory: The Journal of Medical Laboratory, 8(2), 85–94, (2021).
Hidayat, Triwahyuni. T, Zulfian, Ayuningsih. V.L, “Differences in Platelet Index (PDW, MPV, P-LCR, PCT) and Platelet Count Between Primary and Secondary Dengue Infection Patients at Dr. H. Abdul Moeloek Lampung Hospital.” Journal of Health Science, (2021).
Chatterjee. A.B, Matti. M, Kulkarni. V, “Role of platelet parameters in dengue fever in Children.” Pediatr. Oncall 17, 12–15, (2020), https://doi.org/10.7199/ped.oncall.2020.10.
Putri Lintang Kharisma, Annisa Muhyi, Eva Rachmi, “Relationship between Nutritional Status, Age, Gender and Degree of Dengue Infection in Children at Abdul Wahab Sjahranie Hospital Samarinda.” J.Sains Kes, Vol 3, No 3, p-ISSN:2303-0267, e-ISSN:2407-6082, (2021).
Hermawan Diki, “The Relationship between Characteristics of Clients with Dengue Hemorrhagic Fever (DHF) and the Incidence of Dengue Hemorrhagic Fever (DHF) in the Work Area of Puskesmas I East Purwokerto, Banyumas Regency.” Thesis, Muhammadiyah Purwokerto University, (2017).
Umaya. R, “Health Services with the Incidence of Dengue Fever (DHF) in the Working Area of Talang Ubi Pendopo Health Center.” J. Health Science. Society, 4, 262-269, (2013).
Nur Rahmasari S. Tule, Tri Dyah Astuti, “Systematic Review: Identification of Gender and Age Group Factors in Dengue Fever Patients with Thrombocytopenia Case Approach.” Bachelor of Applied Medical Laboratory Technology Study Program, Faculty of Health Sciences, Aisyiyah University Yogyakarta, (2020).
D.P.G. Jananuraga Maharddhika, I Wayan Putu Sutirta Yasa, “Prevalence of Dengue Infection Based on NS1 Antigen Test Results in Patients at Niki Diagnostic Center in 2011-2012.” e-Journal Medika Udayana, (2012).
Asha. J.M, Baiju. N, Susheela Innah. J, Rafi. A, John. B.M, “Comparison of Platelet Indices in Dengue Fever Patients Based on Platelet Transfusion: a Prospective Observational Study in a Tertiary Care Center 12.” 105–111, (2022). https://doi.org/10.4103/ajts.AJTS.
Hasanatul Fitriani, Endah Purnamasari, Firman Arifandi, “The Relationship between Serology Tests and Thrombocyte Count in Dengue Fever Patients at Siti Hajar Islam Hospital, Mataram City September 2020-September 2021 Period.” Junior Medical Jurnal, Volume 1 No. 3, (2022).
Khatri. S, Sabeena. S, Arunkumar. G, Mathew. M, “Utility of Platelet Parameters in Serologically Proven Dengue Cases with Thrombocytopenia.” Indian J, Hematol, Blood Transfus, 34, 703–706, (2018), https://doi.org/10.1007/s12288-018-0924-2.
Fahimi. H, Mohammadipour. M, Haddad Kashani. H, Parvini. F, Sadeghizadeh. M, “Dengue Viruses and Promising Envelope Protein Domain III-based Vaccines.” Appl. Microbiol, Biotechnol, 102, 2977–2996, (2018). https://doi.org/10.1007/s00253-018-8822-y.
Hartoyo. E, “Struktur dan Fungsi Sel Endotel pada Demam Berdarah.” Intrans Publishing, (2015). Malang.
Nisalak. A, “ Laboratory Diagnosis of Dengue Virus Infections.” Southeast Asian J. Trop. Med. Public Health 46, 55–76, (2017).
Nugraha. G, “Panduan Pemeriksaan Laboratorium Hematologi Dasar 2nd ed.” (2017). Trans Info Media, Jakarta.
Kesuma. S, “Uji Diagnosis NS1 , IgG dan IgM Dengue Metode Immunokromatografi dan Elisa, (2022). 7, 72–85.
St. John. A. L, Rathore. A.P.S, “ Adaptive Immune Responses to Primary and Secondary Dengue Virus Infections. Nat. Rev. Immunol. (2019) 194 19, 218–230. https://doi.org/10.1038/s41577-019-0123-x.
Rosa Dwi Wahyuni, M. Sabir, “Karakteristik Penderita Demam Berdarah Dengue (DBD) di Rumah Sakit Wahidin Sudirohusodo Makassar Periode Januari – Desember 2010. No.XIV Edisi Oktober (2011). http://jurnal:untad.ac.id/jurnal/index.php//NSP/article/download/2803/1897.
Yaumil Fachni Tandjungbulu, Zulfikar Ali Hasan, Haerani, “Compatibility Test Diagnostic Kits of Nonstructural-1 Dengue Antigen Immunocromatography Method of Patient’s Serum with Suspect Dengue Haemorrhagic Fever. Urban Health, Vol. 2, No.1, (2019). https://journal.poltekkes-mks.ac.id/ojs2/index.php/Prosiding/article/view/1402/848.
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