Efek Asupan Antioksidan Kaya Enzim Cytochrome P450 2E1 (CYP2E1) Terhadap Kadar Leukosit

Baharuddin M. Subandi; Suardi Zurimi; Abdul Rohim Tualeka; Nanda Bella Puspitaloka

doi:10.32382/medkes.v19i2.1051

Authors

Baharudin M. Subandi Poltekkes Kemenkes Maluku
Suardi Zurimi Poltekkes Kemenkes Maluku
Abdul Rohim Tualeka Fakultas Kesehatan Masyarakat Universitas Airlangga
Nanda Bella Puspitaloka Fakultas Kesehatan Masyarakat Universitas Airlangga

DOI:

https://doi.org/10.32382/medkes.v19i2.1051

Keywords:

leukosit, antioksidan, CYP2E1

Abstract

The human body has an enzymatic antioxidant system, but its capacity is not always sufficient when the amount of free radicals is excessive. The approach of consuming antioxidants rich in the CYP2E1 enzyme is used to help the body detoxify benzene metabolites so they can be excreted from the body. The aim of this study is to analyze the effect of consuming antioxidants rich in the CYP2E1 enzyme on changes in leukocyte levels among workers in the Tambak Osowilangun Surabaya home-based shoe industry. This type of research is a pre-experimental study using a one-group pre-test post-test design. The sample used in this study was total population sampling, consisting of all 27 workers in the Tambak Osowilangun home-based shoe industry. The intervention provided was in the form of antioxidant supplements in powder form, with a dosage of 15 grams, consumed twice a day for 5 consecutive days. Leukocyte levels were measured by taking blood samples from the workers on the first day before the intervention and on the fifth day after the intervention. The results of the study showed changes in the leukocyte levels of all workers after the treatment. There was an increase in the number of workers who reached normal leukocyte levels (92.6%). However, some workers did not reach normal leukocyte levels (7.4%) despite experiencing changes in their leukocyte levels. The conclusion of this study is that the intervention was effective in normalizing the workers' leukocyte levels, particularly in female workers, those of productive age, with normal BMI, non-smokers, those who engage in physical activity/exercise, and those who do not use personal protective equipment (PPE). It is recommended that workers regularly engage in exercise that suits their preferences and body needs, adopt a balanced diet, consume foods rich in antioxidants, use brushes when applying glue, and use standard PPE such as N95 masks, gloves, and aprons..

Keywords: leukocyte count; antioxidant; CYP2E1

References

Kurniawidjaja LM, Fatma L, Mila T, Doni H. Konsep Dasar Toksikologi Industri. Depok: Universitas Indonesia; 2021.

Li X, Yuanyuan G, Xiuling S, Yinghua H, Huiwen Z, Hao B, et al. A cross-sectional survey based on blood VOCs, hematological parameters and urine indicators in a population in Jilin, Northeast China. Environ Geochem Health. 2019;41(2):1599–615.

Michael Gochfeld. Sex Differences in Human and Animal Toxicology: Toxicokinetics. Toxicol Pathol. 2017;45(1):172–89.

Moro AM, Natalia B, Mariele FC, Marilia B, Elisa S, Gabriela G, et al. Biomonitoring of gasoline station attendants exposed to benzene: Effect of gender. Mutat Res - Genet Toxicol Environ Mutagen. 2017;813:1–9.

Darwis D, Mubarak M, Anita S. Risiko Paparan Benzena Terhadap Kandungan Fenol Dalam Urin Pekerja Pengecatan Mobil Di Kecamatan Tampan Kota Pekanbaru Tahun 2017. Din Lingkung Indones. 2018;5(1):40.

Tenchov R, Sasso JM, Wang X, Zhou QA. Aging Hallmarks and Progression and Age-Related Diseases: A Landscape View of Research Advancement. ACS Chem Neurosci. 2024;15(1):1–30.

Saraswati SK, Rahmaningrum FD, Pahsya MNZ, Paramitha N, Wulansari A, Ristantya AR, et al. Literature Review : Faktor Risiko Penyebab Obesitas. Media Kesehat Masy Indones. 2021;20(1):70–4.

Salsabila NN, Indraswari N, Sujatmiko B. Gambaran Kebiasaan Merokok Di Indonesia Berdasarkan Indonesia Family Life Survey 5 (Ifls 5). J Ekon Kesehat Indones. 2022;7(1):13.

Thyfault JP, Bergouignan A. Exercise and metabolic health: beyond skeletal muscle. Diabetologia. 2020;63(8):1464–74.

Martínez de Toda I, González-Sánchez M, Díaz-Del Cerro E, Valera G, Carracedo J, Guerra-Pérez N. Sex differences in markers of oxidation and inflammation. Implications for ageing. Mech Ageing Dev. 2023;211(February).

Ramdiana R, Legiran L. Literature Review: Stres Oksidatif dan Reproduksi Wanita. J Ilmu Kesehat Masy. 2023;12(03):202–14.

Bestari EM, Sudarmaji, Sulistyorini L. Source of Benzene, Characteristics and Hemoglobin Levels of AHASS Mechanical Workers at Kediri City. J Kesehat Lingkung. 2019;11(4):293–9.

Rahmawati A. Mekanisme Terjadinya Inflamasi Dan Stres Oksidatif Pada Obesitas. el–Hayah. 2014;5(1):1.

Wouters K, Gaens K, Bijnen M, Verboven K, Jocken J, Wetzels S, et al. Circulating classical monocytes are associated with CD11c+ macrophages in human visceral adipose tissue. Sci Rep [Internet]. 2017;7(February):1–8. Tersedia pada: http://dx.doi.org/10.1038/srep42665

Jeong HR. Positive Association Between Body Mass Index and Hematologic Parameters , Including RBC , WBC and Platelet Count , in Korean Children and Adolescent. 2021;1–22.

Salem E, El-Garawani I, Allam H, El-Aal BA, Hegazy M. Genotoxic effects of occupational exposure to benzene in gasoline station workers. Ind Health. 2018;56(2):132–40.