Deteksi Gen Resistensi Karbapenemase Dan Metallo-Β-Lactamase Pada Acinetobacter Baumannii
DOI:
https://doi.org/10.32382/jmak.v15i1.631Kata Kunci:
Acinetobacter baumannii, Karbapenem, MBL, polymerase chain reactionAbstrak
Peningkatan resistensi terhadap antibiotik golongan karbapenem merupakan salah satu fenomena yang harus diwaspadai. Acinetobacter baumannii merupakan salah satu bakteri penyebab infeksi berat yang didapatkan di rumah sakit. Penelitian ini bertujuan untuk mendeteksi gen intrinsik dan gen yang membawa resistensi obat golongan karbapenem terhadap A. baumannii. Penelitian ini dilaksanakan di Laboratorium HUM-RC RSPTN Universitas Hasanuddin Makassar. Desain penelitian ini adalah deskriptif eksperimental laboratorium dengan jumlah sampel 50 isolat yang didapatkan dengan cara total sampling yang dikumpulkan dari RSUP Wahidin Sudirohusodo Makassar. Data yang dianalisis adalah hasil karakteristik isolat, uji sensitivitas dengan menggunakan Vitek-2, uji genotipe gen intrinsik OXA-51 dan blaIMP-1 dengan menggunakan Polymerase Chain Reaction. Hasil penelitian menunjukkan bahwa dari total 50 isolat A. baumannii, ditemukan paling banyak pada sampel sputum sejumlah 30 isolat (60%). Dari total 50 sampel uji sensitivitas antibiotik, isolat A. baumanni paling banyak resisten terhadap meropenem 14 isolat (28%) kemudian imipenem 9 isolat (18%), dan Doripenem 9 isolat (18%). Gen intrinsik OXA-51 ditemukan pada semua isolat sampel sedangkan hanya satu isolat dari A. baumannii yang mampu memproduksi enzim metallo-β-Lactamase (MBL) dan membawa gen blaIMP-1 yaitu pada sampel urine yang seluruhnya resisten terhadap ketiga golongan obat karbapenem.
Kata Kunci : Acinetobacter baumannii, Karbapenem, MBL, Polymerase Chain Reaction
Referensi
Adams, M. D., Nickel, G. C., Bajaksouzian, S., Lavender, H., Murthy, A. R., Jacobs, M. R., & Bonomo, R. A. (2009). Resistance to colistin in Acinetobacter baumannii associated with mutations in the PmrAB two-component system. Antimicrobial agents and chemotherapy, 53(9), 3628-3634.
Aghamiri, S., Amirmozafari, N., Fallah Mehrabadi, J., Fouladtan, B., & Hanafi Abdar, M. (2016). Antibiotic resistance patterns and a survey of metallo-β-lactamase genes including Bla-IMP and Bla-VIM types in Acinetobacter baumannii isolated from hospital patients in Tehran. Chemotherapy, 61(5), 275-280.
Al Samawi, M. S., Khan, F. Y., Eldeeb, Y., Almaslamani, M., Alkhal, A., Alsoub, H., & Hashim, S. (2016). Acinetobacter infections among adult patients in Qatar: a 2-year hospital-based study. Canadian Journal of Infectious Diseases and Medical Microbiology, 2016.
Begum, S., Hasan, F., Hussain, S., & Shah, A. A. (2013). Prevalence of multi drug resistant Acinetobacter baumannii in the clinical samples from Tertiary Care Hospital in Islamabad, Pakistan. Pakistan journal of medical sciences, 29(5), 1253.
Bush, K., & Bradford, P. A. (2019). Interplay between β-lactamases and new β-lactamase inhibitors. Nature Reviews Microbiology, 17(5), 295-306.
Evans, B. A., & Amyes, S. G. (2014). OXA β-lactamases. Clinical microbiology reviews, 27(2), 241-263.
Fang, F., Wang, S., Dang, Y. X., Wang, X., & Yu, G. Q. (2016). Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China. Genetics and Molecular Research, 15(1).
Gonzalez-Bello, C., Rodríguez, D., Pernas, M., Rodriguez, A., & Colchon, E. (2019). β-Lactamase inhibitors to restore the efficacy of antibiotics against superbugs. Journal of medicinal chemistry, 63(5), 1859-1881.
Howard, A., O’Donoghue, M., Feeney, A., & Sleator, R. D. (2012). Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence, 3(3), 243-250.
Jabalameli, F., Taki, E., Emaneini, M., & Beigverdi, R. (2018). Prevalence of metallo-β-lactamase-encoding genes among carbapenem-resistant Pseudomonas aeruginosa strains isolated from burn patients in Iran. Revista da Sociedade Brasileira de Medicina Tropical, 51, 270-276.
Kempf, M., & Rolain, J. M. (2012). Emergence of resistance to carbapenems in Acinetobacter baumannii in Europe: clinical impact and therapeutic options. International journal of antimicrobial agents, 39(2), 105-114.
Latifah, R. (2014). Deteksi enzim karbapenemase dan gen pengkodenya pada isolat Pseudomonas aeruginosa dan Acinetobacter baumanii resisten karbapenem di ICU-RSUPN Cipto Mangunkusumo tahun 2011 (Tesis). FK-UI, Jakarta.
Lee, C. R., Lee, J. H., Park, M., Park, K. S., Bae, I. K., Kim, Y. B., ... & Lee, S. H. (2017). Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options. Frontiers in cellular and infection microbiology, 7, 55.
Nirwati, H., Hakim, M. S., Darma, S., Mustafa, M., & Nuryastuti, T. (2018). Detection of blaoxa genes and identification of biofilm-producing capacity of Acinetobacter baumannii in a tertiary teaching hospital, Klaten, Indonesia. Med J Malaysia, 73(5), 291.
Nigro, S. J., & Hall, R. M. (2018). Does the intrinsic oxaAb (bla OXA-51-like) gene of Acinetobacter baumannii confer resistance to carbapenems when activated by ISAba1?. Journal of Antimicrobial Chemotherapy, 73(12), 3518-3520.
Perez, F., Hujer, A. M., Hujer, K. M., Decker, B. K., Rather, P. N., & Bonomo, R. A. (2007). Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrobial agents and chemotherapy, 51(10), 3471-3484.
Rezaei, A., Fazeli, H., Moghadampour, M., Halaji, M., & Faghri, J. (2018). Determination of antibiotic resistance pattern and prevalence of OXA-type carbapenemases among Acinetobacter baumannii clinical isolates from inpatients in Isfahan, central Iran. Infez Med, 26(1), 61-66.
Simo Tchuinte, P. L., Rabenandrasana, M. A. N., Kowalewicz, C., Andrianoelina, V. H., Rakotondrasoa, A., Andrianirina, Z. Z., ... & Collard, J. M. (2019). Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar. Antimicrobial Resistance & Infection Control, 8, 1-9.
Ying, C., Li, Y., Wang, Y., Zheng, B., & Yang, C. (2015). Investigation of the molecular epidemiology of Acinetobacter baumannii isolated from patients and environmental contamination. The Journal of antibiotics, 68(9), 562-567.
