Sensitivity and Specificity of Rapid Antigen Antigen Examination for COVID-19 Immunochromatography Method at the Dadi Regional Special Hospitals
DOI:
https://doi.org/10.32382/jmak.v14i2.247Kata Kunci:
RA COVID-19, RT-PCR, SARS Cov-2, Sensitivity, SpecificityAbstrak
Currently COVID-19 is still in pandemic status so the transmission of this disease needs to be controlled. One of the government's efforts is to screen people suspected of COVID-19 with laboratory examinations using RT-PCR, but this is hampered by long test times. For this reason, the Rapid Antigen COVID-19 (RA COVID-19) immunochromatography (IT) method is used which is short in time and uses simple equipment. This research is a laboratory observational research which aims to determine the sensitivity and specificity of the RA COVID-19 method at RSKD Dadi, South Sulawesi Province. The research sample was 50 suspected COVID-19 patients being treated at RSKD Dadi, South Sulawesi Province and then nasopharyngeal swab samples were taken from these patients. The 50 swab samples were examined using the RA COVID-19 method and the Real Time Polymerase Chain Reaction (RT-PCR) method. Location The research was carried out at the RSKD Dani laboratory, South Sulawesi Province for 6 months (July-December 2022). The research results showed that the RA COVID-19 used at RSKD Dadi, South Sulawesi Province as a COVID-19 screening method for suspected COVID-19 patients had a sensitivity of 87% and a specificity of 97%. Conclusion: RA COVID-19 used at RSKD Dadi, South Sulawesi Province can be used as a screening method, but the results still need to be confirmed using the RT-PCR method
Referensi
Agustina, A. S., & Fajrunni’mah, R. (2020). Perbandingan Metode RT-PCR dan Tes Rapid Antibodi untuk Deteksi COVID-19. Jurnal Kesehatan Manarang, 6(Khusus), 47. https://doi.org/10.33490/jkm.v6ikhusus.317
Aranha, C., Patel, V., Bhor, V., & Gogoi, D. (2021). Cycle threshold values in RT-PCR to determine dynamics of SARS-CoV-2 viral load: An approach to reduce the isolation period for COVID-19 patients. Journal of Medical Virology, 93(12), 6794–6797. https://doi.org/10.1002/jmv.27206
Azer, S. A. (2020). COVID-19: pathophysiology, diagnosis, complications and investigational therapeutics. New Microbes and New Infections, 37(M), 100738. https://doi.org/10.1016/j.nmni.2020.100738
Badan Nasional Penanggulangan Bencana. (2020). Cegah Penularan COVID-19 Dengan Membatasi Keramaian. Retrieved from Badan Nasional Penanggulangan Bencana:
Evgenii Gusev, Alexey Sarapultsev, Liliya Solomatina, V. C. (2022). SARS-CoV-2-Specific Immune Response and the Pathogenesis of COVID-19. Molecular Sciences. https://doi.org/10.3390/ijms23031716
Febri Fitriani, & RA Fadilla. (2020). Pengaruh Senam Diabetes Terhadap Penurunan Kadar Gula Darah Pada Pasien Diabetes Mellitus. Jurnal Kesehatan Dan Pembangunan, 10(19), 1–7. https://doi.org/10.52047/jkp.v10i19.54
Giovanetti, M., Benedetti, F., Campisi, G., Ciccozzi, A., Fabris, S., Ceccarelli, G., Tambone, V., Caruso, A., Angeletti, S., Zella, D., & Ciccozzi, M. (2021). Evolution patterns of SARS-CoV-2: Snapshot on its genome variants. Biochemical and Biophysical Research Communications, 538(xxxx), 88–91. https://doi.org/10.1016/j.bbrc.2020.10.102
Hu, B., Guo, H., Zhou, P., & Shi, Z. L. (2021). Characteristics of SARS-CoV-2 and COVID-19. Nature Reviews Microbiology, 19(3), 141–154. https://doi.org/10.1038/s41579-020-00459-7
Kadam, S. B., Sukhramani, G. S., Bishnoi, P., Pable, A. A., & Barvkar, V. T. (2021). SARS-CoV-2, the pandemic coronavirus: Molecular and structural insights. Journal of Basic Microbiology, 61(3), 180–202. https://doi.org/10.1002/jobm.202000537
Martini, M., & Mendrofa, H. K. (2021). Hubungan Usia dan Jenis Kelamin Dengan Penderita COVID-19 Di Rumah Sakit Aminah Kota Tangerang. MAHESA : Malahayati Health Student Journal, 1(4), 411–416. https://doi.org/10.33024/mahesa.v1i4.5188
Md. Mahfuzur Rahman, a Ananya Ferdous Hoque, a Yeasir Karim, a Zannat Kawser, b Abu Bakar Siddik, b Mariya Kibtiya Sumiya, a Ayesha Siddika, a Md. Shaheen Alam, a Ahmed Nawsher Alam, c Muntasir Alam, a Mohammad Enayet Hossain, a Sayera Banu, a Firdausi Qa, a and M. Z. R. (2021). Clinical evaluation of SARS-CoV-2 antigen-based rapid diagnostic test kit for detection of COVID-19 cases in Bangladesh. Heliyon, 7(11). https://doi.org/10.1016/j.heliyon.2021.e08455
Munne, K., Bhanothu, V., Mayekar, A., Birje, S., Bhor, V., Patel, V., Mahale, S. D., & Pande, S. S. (2021). A retrospective analysis of COVID-19 diagnosis results obtained by rapid antigen tests and RT-PCR: Implications for disease management. Indian Journal of Medical Microbiology, 39(4), 537–539. https://doi.org/10.1016/j.ijmmb.2021.05.006
Pusparini, P. (2020). Tes serologi dan polimerase chain reaction (PCR) untuk deteksi SARS-CoV-2/COVID-19. Jurnal Biomedika Dan Kesehatan, 3(2), 46–48. https://doi.org/10.18051/jbiomedkes.2020.v3.46-48
Rachmawati, S., Windusari, Y., & Hasyim, H. (2022). Perbandingan Hasil COVID-19 Metode Rapid Antigen Test dengan PCR: Literature Review. Jurnal Ilmiah Universitas Batanghari Jambi, 22(3), 1706. https://doi.org/10.33087/jiubj.v22i3.2767
World Health Organization. (2021). Coronavirus disease (COVID-19) Pandemic–Emergency Use Listing Procedure (EUL) open for in vitro diagnostics.
Yamayoshi, S., Sakai-Tagawa, Y., Koga, M., Akasaka, O., Nakachi, I., Koh, H., Maeda, K., Adachi, E., Saito, M., Nagai, H., Ikeuchi, K., Ogura, T., Baba, R., Fujita, K., Fukui, T., Ito, F., Hattori, S. I., Yamamoto, K., Nakamoto, T., … Kawaoka, Y. (2020). Comparison of rapid antigen tests for COVID-19. Viruses, 12(12), 1–9. https://doi.org/10.3390/v12121420
Yulianti, R., & Astari, R. (2020). Jurnal Kesehatan Jurnal Kesehatan. Jurnal Kesehatan, 8(1), 10–15.
Yuniarti, L., Tejasari, M. and Purbaningsih, W. (2020). Bunga Rampai Artikel Penyakit Virus Korona (COVID-19) Editor : Titik Respati. Kopidpedia, 203–215.
